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Objective To investigate the change of coagulation indexes in the patients with bee sting injury .Methods A total of 294 cases of bee sting injury were selected as the study subjects and divided into the mild bee sting injury group (A ,1 -2 sites) , moderate bee sting injury group(B ,3-10sites) ,severe bee sting injury group(C ,11-20sites) and extremely severe bee sting injury group(D ,≥21sites) according to the number of sting site .Meanwhile 40 healthy people were selected as the control group .The changes of prothrombin time(PT),internation alnormalized ratio(INR),activated partial thromboplastin time(APTT)and fibrinogen(FIB) were compared among groups .Results PT and APTT in the severe bee sting injury group and extremely severe bee sting injury group were significantly prolonged within 4-9 h after bee sting injury compared with the control group (P＜0 .05) .APTT , PT and INR had obviously dose effect positive correlation with bee sting sites (r=0 .583 ,0 .340 ,0 .327 ,P＜0 .01) .Conclusion APTT is closely correlated with bee sting sites and seeing doctor time ,which can serve as the effective monitoring indexes in the treatment process of bee sting injury .
ABSTRACT
<p><b>OBJECTIVE</b>To assess the association of IFNG gene polymorphisms with preeclampsia among pregnant woman from Shaanxi Province.</p><p><b>METHODS</b>Genomic DNA was extracted from peripheral blood samples collected from 280 patients with preeclampsia and 344 healthy pregnant women. Five tag single nucleotide polymorphisms (SNPs) of the IFNG gene (rs2069705, rs2430561, rs1861493, rs2069718, and rs2193050) were genotyped with a SNaPshot method. Genotypic and allelic frequencies were evaluated with a Chi square test. Genotype data was corrected by Logistic regression for body mass index and age. The level of IFN-gamma was determined with an ELISA assay.</p><p><b>RESULTS</b>The distribution of five tag SNPs all conformed to Hardy-Weinberg equilibrium (P> 0.05). Significant association with preeclampsia was found with the T allele of rs2430561 (OR=1.54, 95% CI:1.15-2.09, P=6.99× 10), under a dominant model (OR=3.77, 95% CI: 1.09-13.29, P=0.029) and a recessive model (OR=1.53, 95% CI:1.09-2.15, P=0.018). For the patient group, the IFN-gamma level of those with a TT genotype for rs2430561 was significantly higher than those with an AA or AT genotype [(13.69± 0.79) pg/mL vs. (13.11± 1.56) pg/mL, P< 0.05].</p><p><b>CONCLUSION</b>Polymorphism of the rs2430561 locus of the IFNG gene is associated with increased risk for preeclampsia as well as serum level of IFN-gamma among pregnant woman from Shaanxi. The role of the IFNG gene in the regulation of preeclampsia requires further investigation.</p>
Subject(s)
Adult , Female , Humans , Pregnancy , Genetic Predisposition to Disease , Genotype , Interferon-gamma , Blood , Genetics , Logistic Models , Polymorphism, Single Nucleotide , Pre-Eclampsia , GeneticsABSTRACT
Objective To investigate the changes of WBC count and neutrophil ratio (NEU% ) in the patients with bee sting inju‐ry .Methods According to the number of bee sting and different time going to hospital ,the WBC count and NEU% in 315 cases of bee sting injury were analyzed and compared with those in healthy population .Results The changes of WBC count and NEU% had statistically significant difference between the patients with bee sting injury and the healthy people (P< 0 .05) .The changes of WBC count and NEU% had statistically significant difference between the patients with more than 20 bee sting injuries and the pa‐tients with less than 3 bee sting injuries(P<0 .05) .The WBC count had obvious difference between the patients with treatment within 3 h after bee sting and the patients with treatment at other time (P<0 .05) .Conclusion The WBC count and NEU% are obviously increased after bee sting ,which is closely correlated with the bee sting injury number and the time going to hospital .
ABSTRACT
Objective Previous studies have shown that genetic variants in the interferons regulatory factor 5 (IRF5) gene are associated with rheumatoid arthritis (RA) in European and Japanese, but not found in Han Chinese. We conducted this study to investigate whether genetic variants in the IRF5 gene are associated with RA in ShaanXi Han Chinese population. Methods This study was collected 576 RA patients and 768 normal controls. Six IRF5 gene polymorphisms (rs729302, rs2004640, rs752637, rs3807306, rs10954213 and rs2280714) were genotyped by the SNaPshot method. T-test and χ2 test were used for statistic analysis. The genotype and allele frequencies were evaluated using the chi square tests. Genotyping data were adjusted by Logistic regression method by age and gender. The linkage disequilibrium (LD) block structure was examined using Hapview 4.2 software. Results Six SNPs inspected complied with Hardy-weinberg equilibrium (P>0.05). Two SNPs were significantly associated with RA: rs729302 A risk allele [OR=1.29, 95%CI (1.10, 1.50), P=5.57×10-3];dominant model [OR=1.58, 95%CI (1.10, 2.27), P=0.024], recessive model [OR=1.31, 95%CI (1.17, 1.64), P=0.028]. rs2004640 T risk allele [OR=1.28, 95%CI (1.08, 1.54), P=0.039]; dominant model [OR=1.27, 95%CI (1.03, 1.58), P=0.036]. In addition, there was no significant difference in rs752637, rs3807306, rs10954213 and rs2280714 SNPs between RA group and control and genotyped polymorphisms were significantly associated with RA susceptibility. Conclusion The present study confirm that rs729302 and rs2004640 in the IRF5 gene is significantly associated with increased risk of RA in ShaanXi Han Chinese population.